Anticorps Polyclonal de lapin anti-TBP

TBP Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 22246-1-AP

Synonymes

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

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Galerie de données de validation

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  WB analysis of Jurkat using 22246-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22246-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 22246-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22246-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 22246-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 22246-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TBP antibody 22246-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules COLO 320, cellules HeLa, cellules SKOV-3, tissu de côlon de souris
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

22246-1-AP cible TBP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBP Protéine recombinante Ag17740
• Nom complet: TATA box binding protein
• Masse moléculaire calculée: 338 aa, 38 kDa
• Poids moléculaire observé: mouse/rat 33-36 kDa and human 37-43kDa
• Numéro d’acquisition GenBank: BC110341
• Symbole du gène: TBP
• Identification du gène (NCBI): 6908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBP, TATA box-binding protein, is a DNA-binding subunit of TFIID, which is a multisubunit complex essential for the expression of most protein-encoding genes. The well-conserved C-terminal domains of 180 aa is both necessary and sufficient for interaction with DNA and for assembly of the basal transcription apparatus.The N terminus of TBP modulates the DNA-binding activity of the C terminus of the protein. Once binds to the TATA box mediated by TBP, TFIID initiate the transcription step of the pre-initiation complex(PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This is a rabbit polyclonal antibody raised against part of N-terminal chain of human TBP, and specially recognize a ~46 kDa band.

Publications

Species	Application	Title
mouse	WB	Breast Cancer; Isoforms of IDH in breast carcinoma: IDH2 as a potent prognostic factor associated with proliferation in estrogen-receptor positive cases.; Authors - Hiroyuki Minemura
human	WB	Mol Med Rep; Subcellular localization of DJ-1 in human HL-60 leukemia cells in response to diallyl disulfide treatment.; Authors - Qingye Li

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