Anticorps Monoclonal anti-TBP
TBP Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, porc, rat, souris
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugaison
Non conjugué
CloneNo.
2H3B2
N° de cat : 66166-1-Ig
Synonymes
"TBP Antibodies" Comparison
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Applications testées
| Résultats positifs en WB | cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules NIH/3T3, tissu hépatique de porc | 
| Résultats positifs en IP | cellules HEK-293 | 
| Résultats positifs en IHC | tissu de cancer du foie humain,  tissu de cancer du sein humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. | 
| Résultats positifs en IF/ICC | cellules A431, cellules HeLa | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 | 
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunohistochimie (IHC) | IHC : 1:325-1:1300 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 23 publications below | 
| IF | See 1 publications below | 
Informations sur le produit
66166-1-Ig cible TBP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris
| Réactivité | Humain, porc, rat, souris | 
| Réactivité citée | rat, Humain, porc, souris | 
| Hôte / Isotype | Mouse / IgG2a | 
| Clonalité | Monoclonal | 
| Type | Anticorps | 
| Immunogène | TBP Protéine recombinante Ag12383 | 
| Nom complet | TATA box binding protein | 
| Masse moléculaire calculée | 338 aa, 38 kDa | 
| Poids moléculaire observé | mouse/rat 33-36 kDa and human 37-43kDa | 
| Numéro d’acquisition GenBank | BC110341 | 
| Symbole du gène | TBP | 
| Identification du gène (NCBI) | 6908 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par protéine A | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for TBP antibody 66166-1-Ig | Download protocol | 
| IHC protocol for TBP antibody 66166-1-Ig | Download protocol | 
| IF protocol for TBP antibody 66166-1-Ig | Download protocol | 
| IP protocol for TBP antibody 66166-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Genome Res Ligand-induced native G-quadruplex stabilization impairs transcription initiation. | ||
| PLoS Biol Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22. | ||
| Virulence Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions. | ||
| Biochem Pharmacol PPARα regulates the expression of human arylacetamide deacetylase involved in drug hydrolysis and lipid metabolism. | ||
| Front Oncol TFEB Promotes Prostate Cancer Progression via Regulating ABCA2-Dependent Lysosomal Biogenesis. | ||
| Mol Cancer Res Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics. | 
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Aditya (Verified Customer) (07-15-2025) | Excellent, works as advertised 
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