Anticorps Monoclonal anti-TBP

TBP Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(26)

CloneNo.

2H3B2

N° de cat : 66166-1-Ig

Synonymes

TATA sequence binding protein, TATA box factor, TATA box binding protein, TATA binding factor, GTF2D1


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  • Western Blot (WB) analysis of various lysates using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis using 66166-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis using 66166-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis of HEK-293 using 66166-1-Ig

    Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-Ig at a dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis of pig liver using 66166-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TBP Monoclonal antibody (66166-1-Ig)

    IP experiment of HEK-293 using 66166-1-Ig

    IP result of anti-TBP (IP:66166-1-Ig, 3ug; Detection:66166-1-Ig 1:800) with HEK-293 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human liver cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human liver cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    I After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBP Monoclonal antibody (66166-1-Ig)

    IF Staining of HeLa using 66166-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66166-1-Ig(TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TBP Monoclonal antibody (66166-1-Ig)

    IF Staining of A431 using 66166-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBP antibody (66166-1-Ig, Clone: 2H3B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

"TBP Antibodies" Comparison

View side-by-side comparison of TBP antibodies from other vendors to find the one that best suits your research needs.

Applications testées

Résultats positifs en WBcellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules NIH/3T3, tissu hépatique de porc
Résultats positifs en IPcellules HEK-293
Résultats positifs en IHCtissu de cancer du foie humain, tissu de cancer du sein humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules A431, cellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:325-1:1300
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66166-1-Ig cible TBP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéerat, Humain, porc, souris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène TBP Protéine recombinante Ag12383
Nom complet TATA box binding protein
Masse moléculaire calculée 338 aa, 38 kDa
Poids moléculaire observé mouse/rat 33-36 kDa and human 37-43kDa
Numéro d’acquisition GenBankBC110341
Symbole du gène TBP
Identification du gène (NCBI) 6908
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).

Protocole

Product Specific Protocols
WB protocol for TBP antibody 66166-1-IgDownload protocol
IHC protocol for TBP antibody 66166-1-IgDownload protocol
IF protocol for TBP antibody 66166-1-IgDownload protocol
IP protocol for TBP antibody 66166-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Genome Res

Ligand-induced native G-quadruplex stabilization impairs transcription initiation.

Authors - Conghui Li
mouseWB

PLoS Biol

Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22.

Authors - Yu Chen
humanWB

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
humanWB

Biochem Pharmacol

PPARα regulates the expression of human arylacetamide deacetylase involved in drug hydrolysis and lipid metabolism.

Authors - Tomomi Morikawa
humanWB

Front Oncol

TFEB Promotes Prostate Cancer Progression via Regulating ABCA2-Dependent Lysosomal Biogenesis.

Authors - Xuejin Zhu
humanWB

Mol Cancer Res

Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics.

Authors - Olesja Popow

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Aditya (Verified Customer) (07-15-2025)

Excellent, works as advertised

  • Applications: Western Blot
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