"TBP Antibodies" Comparison
View side-by-side comparison of TBP antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | mouse testis tissue, human breast cancer tissue, human colon tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22006-1-AP targets TBP in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12383 Product name: Recombinant human TBP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-338 aa of BC110341 Sequence: MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT Predict reactive species |
| Full Name | TATA box binding protein |
| Calculated Molecular Weight | 338 aa, 38 kDa |
| Observed Molecular Weight | mouse/rat 33-36 kDa and human 37-43kDa |
| GenBank Accession Number | BC110341 |
| Gene Symbol | TBP |
| Gene ID (NCBI) | 6908 |
| RRID | AB_10951514 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20226 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TBP antibody 22006-1-AP | Download protocol |
| IP protocol for TBP antibody 22006-1-AP | Download protocol |
| WB protocol for TBP antibody 22006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TBP Polyclonal antibody (Cat# 22006-1-AP) has 98 citations appearing in journals including Nature Communications, Cell Metabolism, Molecular Cell, Cancer Research, Hepatology, Developmental Cell, Journal of Biological Chemistry, Genome Biology, and FEBS Journal, among others. Associated research fields span transcription and RNA biology, oncology (hepatocellular, lung, breast, colorectal, and ovarian cancers), inflammation and immune signaling, metabolism, neurology, epigenetics, cell death, and toxicology.
| Species | Application | Title |
|---|---|---|
Cell Metab Nicotinic acid riboside maintains NAD+ homeostasis and ameliorates aging-associated NAD+ decline | ||
Cancer Res DNA of neutrophil extracellular traps binds TMCO6 to impair CD8+ T-cell immunity in hepatocellular carcinoma | ||
Drug Resist Updat Acetate utilization promotes hormone therapy resistance in prostate cancer through neuroendocrine differentiation | ||
Hepatology Steatotic hepatocytes release mature VLDL via methionine and tyrosine metabolism in a Keap1-Nrf2 dependent manner. | ||
Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke |
Reviews
What customers say
The single review for this product is a 5-star rating from a user who tested it by Western Blot at a 1:2000 dilution on human white and brown adipocyte cells. The reviewer reported that the antibody worked very well in these cell types, indicating strong performance and reliable detection in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mi (Verified Customer) (06-25-2023) | This antibody works very well in human white and brown adipocyte cells.
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