TBP Polyclonal antibody

• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of COLO 320 using 22006-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22006-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22006-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 22006-1-AP

  IP result of anti-TBP (IP:22006-1-AP, 4ug; Detection:22006-1-AP 1:500) with COLO 320 cells lysate 2560 ug.

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  IHC staining of mouse testis using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22006-1-AP (TBP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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TBP Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 22006-1-AP

Publications(83)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat and More (4)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: mouse testis tissue, human breast cancer tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 77 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• ChIP: See 1 publications below

Product Information

22006-1-AP targets TBP in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, Rat samples.

• Tested Reactivity: human, mouse, Rat
• Cited Reactivity: human, mouse, rat, pig, monkey, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBP fusion protein Ag12383
• Full Name: TATA box binding protein
• Calculated Molecular Weight: 338 aa, 38 kDa
• Observed Molecular Weight: mouse/rat 33-36 kDa and human 37-43kDa
• GenBank Accession Number: BC110341
• Gene Symbol: TBP
• Gene ID (NCBI): 6908
• RRID: AB_10951514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20226
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.

Publications

Species	Application	Title
mouse	WB	Hepatology; Steatotic hepatocytes release mature VLDL via methionine and tyrosine metabolism in a Keap1-Nrf2 dependent manner.; Authors - Akitoshi Sano
mouse	WB,IP,IF	Redox Biol; Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke; Authors - Haiyun Guo
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	Cancer Res; DNA of neutrophil extracellular traps binds TMCO6 to impair CD8+ T-cell immunity in hepatocellular carcinoma; Authors - Mengjia Song
human	WB	Elife; KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.; Authors - Yool Lee
human	WB	J Hazard Mater; ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.; Authors - Xuan Lai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (06-25-2023)

This antibody works very well in human white and brown adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human adipocytes