TBP Polyclonal antibody

TBP Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22006-1-AP

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID



Tested Applications

Positive WB detected inCOLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22006-1-AP is TBP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, monkey, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBP fusion protein Ag12383
Full Name TATA box binding protein
Calculated molecular weight 338 aa, 38 kDa
Observed molecular weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank accession numberBC110341
Gene symbol TBP
Gene ID (NCBI) 6908
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.

Protocols

Product Specific Protocols
WB protocol for TBP antibody 22006-1-APDownload protocol
IHC protocol for TBP antibody 22006-1-APDownload protocol
IP protocol for TBP antibody 22006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Front Immunol

Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4.

Authors - Mudan Zhang
mouseWB

Front Cell Neurosci

IRAK-M Deficiency Exacerbates Ischemic Neurovascular Injuries in Experimental Stroke Mice.

Authors - Chenfei Lyu
monkeyWB

Am J Physiol Cell Physiol

Expansion of p63-positive CCSP-positive epithelial cells and altered expressions of p63 isoforms in the lung as novel features of aging.

Authors - Jutaro Fukumoto
mouseWB

Cell Rep

Ets1-Mediated Acetylation of FoxO1 Is Critical for Gluconeogenesis Regulation during Feed-Fast Cycles.

Authors - Kai Li
humanWB

Cell Cycle

A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.

Authors - Yanan Wang
mouseWB

J Autoimmun

Interleukin-1 receptor associated kinase (IRAK)-M -mediated type 2 microglia polarization ameliorates the severity of experimental autoimmune encephalomyelitis (EAE).

Authors - Baozhu Liu