"TBP Antibodies" Comparison
View side-by-side comparison of TBP antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in
|COLO 320 cells
|Positive IP detected in
|COLO 320 cells
|Positive IHC detected in
|human breast cancer tissue, mouse testis tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:3000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
22006-1-AP targets TBP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, Rat samples.
|human, mouse, Rat
|human, mouse, rat, monkey, pig
|Host / Isotype
|Rabbit / IgG
|TBP fusion protein Ag12383
|TATA box binding protein
|Calculated molecular weight
|338 aa, 38 kDa
|Observed molecular weight
|mouse/rat 33-36 kDa and human 37-43kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mi (Verified Customer) (06-25-2023)
This antibody works very well in human white and brown adipocyte cells.