TBP Polyclonal antibody
TBP Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 22006-1-AP
Synonyms
"TBP Antibodies" Comparison
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Tested Applications
Positive WB detected in | COLO 320 cells |
Positive IP detected in | COLO 320 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 53 publications below |
IF | See 2 publications below |
Product Information
The immunogen of 22006-1-AP is TBP Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, monkey, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TBP fusion protein Ag12383 |
Full Name | TATA box binding protein |
Calculated molecular weight | 338 aa, 38 kDa |
Observed molecular weight | mouse/rat 33-36 kDa and human 37-43kDa |
GenBank accession number | BC110341 |
Gene symbol | TBP |
Gene ID (NCBI) | 6908 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for TBP antibody 22006-1-AP | Download protocol |
IHC protocol for TBP antibody 22006-1-AP | Download protocol |
IP protocol for TBP antibody 22006-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hepatology Steatotic hepatocytes release mature VLDL via methionine and tyrosine metabolism in a Keap1-Nrf2 dependent manner. | ||
Cell Death Dis 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer | ||
J Hazard Mater ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes. | ||
EMBO Rep LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis. |