Anticorps Polyclonal de lapin anti-TBX18

TBX18 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 23237-1-AP

Synonymes

T box 18, T box protein 18, TBX18

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Galerie de données de validation

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  WB analysis of HeLa using 23237-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23237-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23237-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 23237-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23237-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 23237-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 23237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23237-1-AP (TBX18 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 23237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23237-1-AP (TBX18 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 23237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23237-1-AP (TBX18 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 23237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23237-1-AP (TBX18 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu placentaire humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

23237-1-AP cible TBX18 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBX18 Protéine recombinante Ag19258
• Nom complet: T-box 18
• Masse moléculaire calculée: 607 aa, 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC157841
• Symbole du gène: TBX18
• Identification du gène (NCBI): 9096
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The T-box (Tbx) motif is present in a family of genes whose structural features and expression patterns support their involvement in developmental gene regulation. The Tbx gene family are largely conserved throughout metazoan evolution, and these genes code for putative transcription factors that share a uniquely defining DNA-binding domain. Tbx genes are a family of developmental regulators with more than 20 members recently identified in invertebrates and vertebrates. Mutations in Tbx genes are associated with the onset of several human diseases. Our understanding of functional mechanisms of Tbx products has come mainly from the prototypical T/Brachyury, which is a transcription activator. The Tbx genes constitute a family of transcriptional regulatory genes that are implicated in a variety of developmental processes ranging from the formation of germ layers to the organizational patterning of the central nervous system.This antibody specifically reacts with the 65kd TBX18 protein.

Publications

Species	Application	Title
human	IF	EBioMedicine; E2A ablation enhances proportion of nodal-like cardiomyocytes in cardiac-specific differentiation of human embryonic stem cells.; Authors - Xiuya Li
rat	WB	Mol Med Rep; G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region; Authors - Pei Xu