Anticorps Polyclonal de lapin anti-TCF12/HEB

• Phare
• Validé par KD/KO

TCF12/HEB Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 14419-1-AP

Synonymes

TCF12, bHLHb20, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E box binding protein

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Galerie de données de validation

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  WB analysis using 14419-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14419-1-AP (TCF12/HEB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14419-1-AP

  WB result of TCF12/HEB antibody (14419-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCF12/HEB transfected HeLa cells.

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  WB analysis of Raji using 14419-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14419-1-AP (TCF12/HEB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 14419-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14419-1-AP (TCF12/HEB antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14419-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14419-1-AP (TCF12/HEB antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules Raji
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Informations sur le produit

14419-1-AP cible TCF12/HEB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCF12/HEB Protéine recombinante Ag5765
• Nom complet: transcription factor 12
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC050556
• Symbole du gène: TCF12
• Identification du gène (NCBI): 6938
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].

Publications

Species	Application	Title
mouse	IF	Nat Cell Biol; Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators; Authors - Bofeng Liu
human	IHC	Nat Commun; TCF12 is mutated in anaplastic oligodendroglioma.; Authors - Karim Labreche
mouse	IF	Development; A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis.; Authors - Sisu Han
mouse	WB,IF	J Orthop Translat; TCF12 regulates the TGF-β/Smad2/3 signaling pathway to accelerate the progression of osteoarthritis by targeting CXCR4; Authors - Hui Zheng
mouse	WB,IHC,IF	Stem Cells; Tcf12, a Member of Basic Helix-loop-helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation in vitro and in vivo.; Authors - Siqi Yi ; KD Validated
human	WB	iScience; Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.; Authors - Holly Holliday

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