Anticorps Polyclonal de lapin anti-TCF3

• Phare
• Validé par KD/KO

TCF3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 21242-1-AP

Synonymes

E2A, ITF1, Immunoglobulin enhancer-binding factor E12/E47, E47, Class B basic helix-loop-helix protein 21

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Galerie de données de validation

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  WB analysis of Jurkat using 21242-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21242-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 21242-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21242-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 21242-1-AP

  IP result of anti-TCF3 (IP:21242-1-AP, 4ug; Detection:21242-1-AP 1:500) with Jurkat cells lysate 2800ug.

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  IF Staining of HeLa using 21242-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TCF3 antibody (21242-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-3
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21242-1-AP cible TCF3 dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCF3 Protéine recombinante Ag15734
• Nom complet: transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Masse moléculaire calculée: 654 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC110580
• Symbole du gène: TCF3
• Identification du gène (NCBI): 6929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Nat Cell Biol; Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators; Authors - Bofeng Liu
human	WB,CoIP	Cell Signal; Targeting SDCBP2 in acute myeloid leukemia; Authors - Yan Du
human	WB	J Cell Mol Med; The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells.; Authors - Meiju Zhang ; KD Validated
mouse	WB	Mol Immunol; Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development.; Authors - Wei Liang
rat	IP	Life Sci; miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling.; Authors - Ting Liu
mouse	WB	Nat Commun; A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation; Authors - Jianfeng Wu

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