Anticorps Polyclonal de lapin anti-TCF4

• Phare
• Validé par KD/KO

TCF4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

48

N° de cat : 22337-1-AP

Synonymes

bHLHb19, E2 2, ITF 2, ITF2, PTHS, SEF 2, SEF2, SEF2 1, SEF2 1A, SEF2 1B, SL3 3 enhancer factor 2, TCF 4, TCF4, transcription factor 4

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Galerie de données de validation

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  WB analysis using 22337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 22337-1-AP

  IP Result of anti-TCF4 (IP:22337-1-AP, 4ug; Detection:22337-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of mouse brain using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22337-1-AP (TCF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22337-1-AP (TCF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:200.

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  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:200.

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TCF4 antibody (22337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TCF4 antibody (22337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 22337-1-AP (TCF4 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 22337-1-AP (TCF4 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules K-562
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de muscle squelettique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 40 publications below
• IHC: See 5 publications below
• IF: See 8 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below
• ChIP: See 2 publications below

Informations sur le produit

22337-1-AP cible TCF4 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Drosophile, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCF4 Protéine recombinante Ag17813
• Nom complet: transcription factor 4
• Masse moléculaire calculée: 671 aa, 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC125084
• Symbole du gène: TCF4
• Identification du gène (NCBI): 6925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor 4 (TCF4), also known as SEF2, ITF2, E2-2, and ME2, binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription By similarity. Human TCF4 mRNA expression is particularly high in the brain. Usage of numerous 5' exons of the human TCF4 gene potentially yields in TCF4 protein isoforms with 18 different N-termini. In addition, the diversity of isoforms is increased by alternative splicing of several internal exons. Some isoforms contain a bipartite nuclear localization signal and are exclusively nuclear, whereas distribution of other isoforms relies on heterodimerization partners. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression(15547706, 12796377). The scopel of molecular weight of several isoforms is about 60-90 kDa (21789225).

Publications

Species	Application	Title
	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
	WB	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	WB	Nat Commun; Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes.; Authors - Norman L Lehman
	WB,IF,ChIP	Acta Pharmacol Sin; Cardiac-specific deletion of BRG1 ameliorates ventricular arrhythmia in mice with myocardial infarction; Authors - Jing Li
human,Drosophila	WB,IF	Cell Rep; Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons.; Authors - Mitchell D'Rozario ; KD Validated
human	WB	Cancer Lett; TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.; Authors - Sai-Lan Liu ; KD Validated

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