Anticorps Polyclonal de lapin anti-TDRKH specific

• Phare
• Validé par KD/KO

TDRKH specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 13528-1-AP

Synonymes

TDRD2, TDRKH, TDRKH specific, tudor and KH domain containing

---

Galerie de données de validation

• 

  WB analysis of NCCIT using 13528-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 13528-1-AP (TDRKH specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13528-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13528-1-AP (TDRKH specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13528-1-AP

  IP Result of anti-TDRKH specific (IP:13528-1-AP, 4ug; Detection:13528-1-AP 1:7000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13528-1-AP (TDRKH specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13528-1-AP (TDRKH specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13528-1-AP (TDRKH specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13528-1-AP (TDRKH specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 13528-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13528-1-AP (TDRKH specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13528-1-AP

  Immunofluorescent analysis of Hela cells, using TDRKH antibody 13528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellule NCCIT, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 9 publications below
• IP: See 4 publications below

Informations sur le produit

13528-1-AP cible TDRKH specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TDRKH specific Protéine recombinante Ag4422
• Nom complet: tudor and KH domain containing
• Masse moléculaire calculée: 606 aa, 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC032690
• Symbole du gène: TDRKH
• Identification du gène (NCBI): 11022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors.; Authors - Yu H Sun
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
mouse	IF, WB	Nat Commun; PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.; Authors - Deqiang Ding ; KO Validated
mouse	IP	EMBO J; MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA.; Authors - Chia-Ling Hsieh
mouse	WB,IF	EMBO J; Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline.; Authors - Jonathan P Saxe ; KO Validated
mouse	WB,IHC	Proc Natl Acad Sci U S A; Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi.; Authors - Chen Chen C