Anticorps Monoclonal anti-TERF1

TERF1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1D10G4

Avis (1)

N° de cat : 67592-1-Ig

Synonymes

Telomeric repeat-binding factor 1, t TRF1, PIN2, NIMA-interacting protein 2, hTRF1 AS

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Galerie de données de validation

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  WB analysis of K-562 using 67592-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67592-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67592-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

67592-1-Ig cible TERF1 dans les applications de WB, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TERF1 Protéine recombinante Ag12540
• Nom complet: telomeric repeat binding factor (NIMA-interacting) 1
• Masse moléculaire calculée: 439 aa, 50 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC029378
• Symbole du gène: TERF1
• Identification du gène (NCBI): 7013
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Telomeres are nucleoprotein complexes located at chromosome ends that consist of tandem arrays of TTAGGG repeats bound to specific proteins. Telomeres protect chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. TERF1 (telomeric repeat-binding factor 1) binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. It's one component of the shelterin complex, which involved in the regulation of telomere length and protection.

Publications

Species	Application	Title
human	WB,IP,ChIP	Nat Commun; Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence; Authors - Athanasios Siametis

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Refik (Verified Customer) (08-22-2025)

Antibody works really well at a 1:250 - 500 dilution for ICC imaging of 2D and 3D cardiac cells with secondary Alexa Flour antibody at 1:400 - 500 and DAPI for both human and mouse specific cells. Works really well at 1:1000 concentration in western blot for overnight incubation followed by 1:300 secondary antibody incubation for 1 hour for human and mouse tissue.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:250 for ICC and 1:1000 for WB
• Cell Tissue Type: Cardiac myocytes human and mouse derived

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