Anticorps Polyclonal de lapin anti-TERT

TERT Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 27586-1-AP

Synonymes

EC:2.7.7.49, EST2, hEST2, hTERT, TCS1

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Galerie de données de validation

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  WB analysis using 27586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27586-1-AP (TERT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu pulmonaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

27586-1-AP cible TERT dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TERT Protéine recombinante Ag26270
• Nom complet: telomerase reverse transcriptase
• Masse moléculaire calculée: 127 kDa
• Poids moléculaire observé: ~100 kDa
• Numéro d’acquisition GenBank: NM_001193376
• Symbole du gène: TERT
• Identification du gène (NCBI): 7015
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Telomerase reverse transcriptase (TERT) is a catalytic subunit of telomerase. TERT plays a key role in cancer formation, ensuring chromosomal stability by maintaining telomere length, and allowing cells to avert senescence. It constitutes a limiting factor for formation of the telomerase complex in cancer cells. TERT is one of two major components of the larger telomerase complex, which extends telomeres by adding specific short repetitive DNA sequences. Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. The calculated MW of TERT is 127 kDa, 27586-1-AP can detect a band around 100 kDa.

Publications

Species	Application	Title
mouse,human	WB,IF	Chem Biol Interact; Telomerase reverse transcriptase protects against diabetic kidney disease by promoting AMPK/PGC-1a-regulated mitochondrial energy homeostasis; Authors - Nan Ma
human	WB	J Tissue Eng; Establishing an hTERT-driven immortalized umbilical cord-derived mesenchymal stem cell line and its therapeutic application in mice with liver failure; Authors - Qi Chen
mouse	WB	J Inflamm Res; FTO Stabilizes MIS12 to Inhibit Vascular Smooth Muscle Cell Senescence in Atherosclerotic Plaque; Authors - Jingzhao Sun
human	WB	Transl Cancer Res; Effect and mechanism of curcumin on colon cancer cell senescence through early growth response 1 (EGR1); Authors - Dan Xu

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