Anticorps Polyclonal de lapin anti-Testin

Testin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 10258-1-AP

Synonymes

TES

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Galerie de données de validation

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  WB analysis of HEK-293 using 10258-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10258-1-AP (TES antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10258-1-AP (TES antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10258-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10258-1-AP (TES antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10258-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10258-1-AP (TES antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 10258-1-AP

  IP result of anti-TES (IP:10258-1-AP, 4ug; Detection:10258-1-AP 1:1500) with K-562 cells lysate 4000ug.

• 

  IHC staining of human skin cancer using 10258-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10258-1-AP (TES antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 10258-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Testin antibody (10258-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 10258-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Testin antibody (10258-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 10258-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Testin antibody (10258-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HEK-293 using 10258-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10258-1-AP (TES antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules K-562, cellules MCF-7, tissu testiculaire de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en IF/ICC: cellules A549, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

10258-1-AP cible Testin dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Testin Protéine recombinante Ag0379
• Nom complet: testis derived transcript (3 LIM domains)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 38-48 kDa
• Numéro d’acquisition GenBank: BC001451
• Symbole du gène: TES
• Identification du gène (NCBI): 26136
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Testin (TES) is a cytoskeleton-associated protein that localizes along actin stress fibers at cell-cell contact areas and at focal adhesion plaques. It interacts with a variety of cytoskeletal proteins and gets involved in regulation of cell adhesion and migration. TES is a putative tumor suppressor and reduced expression of TES has been reported in various cancers. In addition, TES is a marker for Sertoli-Germ Cell junction and is useful to monitor the disruption of intercellular junctions in the testis.

Publications

Species	Application	Title
human	WB	Mol Neurobiol; Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation; Authors - Ruicheng Yang
mouse,human	IHC	Chem Biol Interact; The JNK/P38 signaling pathway activated by Testin protects the intestinal epithelial barrier against Crohn's disease-like colitis; Authors - Xue Song
human	WB	Front Mol Biosci; Development and Verification of a Hypoxic Gene Signature for Predicting Prognosis, Immune Microenvironment, and Chemosensitivity for Osteosarcoma.; Authors - Fengfeng Wu
mouse	WB	Biol Reprod; The Aryl Hydrocarbon Receptor Is Important for Proper Seminiferous Tubule Architecture and Sperm Development in Mice.; Authors - Hansen Deborah A DA
human	WB	Oncol Lett; Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma.; Authors - Giedrius Steponaitis

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-07-2019)

The antibody works well. The first lane are cells expressing GFP-TES and the second lane is just wildtype cells. The antibody recognizes both endogenous and exogenous TES.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Testin Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10258-1-AP)

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