Anticorps Polyclonal de lapin anti-Transferrin

• Phare
• Validé par KD/KO

Transferrin Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

49

N° de cat : 17435-1-AP

Synonymes

Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, Transferrin

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Galerie de données de validation

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  WB analysis using 17435-1-AP

  human urine lysates were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17435-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17435-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17435-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 17435-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17435-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 17435-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17435-1-AP (Transferrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: échantillon d'urine humaine, tissu rénal de rat, tissu rénal de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 43 publications below
• IHC: See 7 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

17435-1-AP cible Transferrin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Transferrin Protéine recombinante Ag11504
• Nom complet: transferrin
• Masse moléculaire calculée: 698 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC059367
• Symbole du gène: TF
• Identification du gène (NCBI): 7018
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273

Publications

Species	Application	Title
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human,mouse	WB	Sci Adv; The adipocyte-enriched secretory protein tetranectin exacerbates type 2 diabetes by inhibiting insulin secretion from β cells; Authors - Fen Liu
mouse	WB	Biomaterials; The protein corona hampers the transcytosis of transferrin-modified nanoparticles through blood-brain barrier and attenuates their targeting ability to brain tumor.; Authors - Wei Xiao
mouse	WB	Haematologica; Gastrointestinal iron excretion and reversal of iron excess in a mouse model of inherited iron excess.; Authors - Courtney J Mercadante ; KO Validated
human,mouse	WB	Cell Death Dis; Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC; Authors - Wei Xue
human,mouse	WB,IHC	Clin Transl Med; Iron deficiency promotes aortic medial degeneration via destructing cytoskeleton of vascular smooth muscle cells.; Authors - Bowen Li