Anticorps Polyclonal de lapin anti-TFAP2C

• Phare
• Validé par KD/KO

TFAP2C Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, FC, chIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14572-1-AP

Synonymes

ap 2gamma, AP2 GAMMA, ERF1, hAP 2g, TFAP2C, TFAP2G, Transcription factor ERF 1

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Galerie de données de validation

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  WB analysis of HeLa using 14572-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14572-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14572-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14572-1-AP

  IP Result of anti-TFAP2C (IP:14572-1-AP, 4ug; Detection:14572-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14572-1-AP (TFAP2C Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14572-1-AP (TFAP2C Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 14572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 40x lens).

• 

  FC experiment of MCF-7 using 14572-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug TFAP2C antibody (14572-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu placentaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• ChIP: See 2 publications below

Informations sur le produit

14572-1-AP cible TFAP2C dans les applications de WB, IP, IHC, FC, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFAP2C Protéine recombinante Ag5648
• Nom complet: transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC035664
• Symbole du gène: TFAP2C
• Identification du gène (NCBI): 7022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,ChIP	Mol Cancer; CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.; Authors - Tao Jiang ; KD Validated
human	WB	Oncogene; Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.; Authors - Qingcai Meng
human	chIP	Oncogene; TFAP2C regulates carbonic anhydrase XII in human breast cancer.; Authors - Christopher M Franke
human	WB	Front Oncol; TFAP2C-Mediated lncRNA PCAT1 Inhibits Ferroptosis in Docetaxel-Resistant Prostate Cancer Through c-Myc/miR-25-3p/SLC7A11 Signaling.; Authors - Xingkang Jiang
human	WB,IHC	Cancers (Basel); TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB; Authors - Ji Xing ; KD Validated
human	IHC	Nat Commun; Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment; Authors - Xiaojian Lu