Anticorps Polyclonal de lapin anti-TFE3

• Phare
• Validé par KD/KO

TFE3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 14480-1-AP

Synonymes

bHLHe33, RCCP2, TFE3, TFEA, Transcription factor E3

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Galerie de données de validation

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  WB analysis of K-562 using 14480-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14480-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14480-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14480-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14480-1-AP

  IP Result of anti-TFE3 (IP:14480-1-AP, 4ug; Detection:14480-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human renal cell carcinoma using 14480-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14480-1-AP (TFE3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 14480-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14480-1-AP (TFE3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, tissu cérébral de rat, tissu cérébral humain, tissu splénique de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

14480-1-AP cible TFE3 dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFE3 Protéine recombinante Ag5863
• Nom complet: transcription factor binding to IGHM enhancer 3
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC026027
• Symbole du gène: TFE3
• Identification du gène (NCBI): 7030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Publications

Species	Application	Title
human	IF	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Autophagy; Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis.; Authors - Hongfeng Wang
human	WB,IF,IP	Autophagy; PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.; Authors - Bo Wang ; KD Validated
mouse	WB	Cell Discov; Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.; Authors - Yiting Jia
human	WB,IF	Bioorg Med Chem; Terpenoids isolated from Chinese liverworts Lepidozia reptans and their anti-inflammatory activity.; Authors - Siwen Li
human	WB	Biochim Biophys Acta Gen Subj; Anti-cancer effect of marchantin C via inducing lung cancer cellular senescence associated with less secretory phenotype.; Authors - Xiu-Lei Zhang