Anticorps Recombinant de lapin anti-TFF1

TFF1 Recombinant Antibody for FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240424F5

N° de cat : 83467-2-RR

Synonymes

Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein

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Galerie de données de validation

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  FC experiment of MCF-7 using 83467-2-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tff1 Recombinant Antibody (83467-2-RR, Clone:240424F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83467-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83467-2-RR against Human TFF1 were performed. The affinity constant is 0.296 nM.

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Applications testées

• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83467-2-RR cible TFF1 dans les applications de FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TFF1 Protéine recombinante Ag4541
• Nom complet: trefoil factor 1
• Masse moléculaire calculée: 84 aa, 9 kDa
• Numéro d’acquisition GenBank: BC032811
• Symbole du gène: TFF1
• Identification du gène (NCBI): 7031
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.

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