Anticorps Polyclonal de lapin anti-TGFB2/TGF-beta 2

TGFB2/TGF-beta 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 28426-1-AP

Synonymes

TGFB2, TGF-beta 2, Glioblastoma-derived T-cell suppressor factor, G-TSF, Latency-associated peptide

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Galerie de données de validation

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  WB analysis using 28426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28426-1-AP (TGFB2/TGF-beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28426-1-AP (TGF beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 28426-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28426-1-AP (TGF beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 28426-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28426-1-AP (TGF beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28426-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TGF beta 2 antibody (28426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules U2OS, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

28426-1-AP cible TGFB2/TGF-beta 2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TGFB2/TGF-beta 2 Protéine recombinante Ag29126
• Nom complet: transforming growth factor, beta 2
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa, 51 kDa
• Numéro d’acquisition GenBank: NM_001135599
• Symbole du gène: TGFB2
• Identification du gène (NCBI): 7042
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth.

Publications

Species	Application	Title
human	WB	Front Microbiol; Characteristics of Gut Microbiota in Patients With Clear Cell Renal Cell Carcinoma.; Authors - Yang Chen
rat	IF	Front Chem; ADSCs Promote Tenocyte Proliferation by Reducing the Methylation Level of lncRNA Morf4l1 in Tendon Injury.; Authors - Haibo Zhao
human	WB	Life (Basel); MPP7 as a Novel Biomarker of Esophageal Cancer: MPP7 Knockdown Inhibits Esophageal Cancer Cell Migration and Invasion; Authors - Zhaodong Li
human	IF	Cancers (Basel); Profiling the Adrenergic System in Breast Cancer and the Development of Metastasis; Authors - Daniela M. Sousa
mouse	WB	J Ocul Pharmacol Ther; Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function; Authors - Xiaochen Wang
rabbit	WB	Small; MiR-141-3p-Functionalized Exosomes Loaded in Dissolvable Microneedle Arrays for Hypertrophic Scar Treatment; Authors - Sheng Meng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rebecca (Verified Customer) (08-14-2025)

Unfortunately, this antibody did not work in our hands. We attempted to use it for western blot on mouse anterior segment tissue (eyes injected with vector expressing TGFb2).

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse anterior segment

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