Anticorps Polyclonal de lapin anti-TGIF2

TGIF2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11522-1-AP

Synonymes

5 TG 3 interacting factor 2, Homeobox protein TGIF2, TGFB induced factor 2, TGFB induced factor homeobox 2, TGIF2

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Galerie de données de validation

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  WB analysis using 11522-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11522-1-AP (TGIF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 11522-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11522-1-AP (TGIF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11522-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11522-1-AP (TGIF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 11522-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using TGIF2 antibody (11522-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules Daudi, cellules HL-60, cellules Jurkat
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

11522-1-AP cible TGIF2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TGIF2 Protéine recombinante Ag2085
• Nom complet: TGFB-induced factor homeobox 2
• Masse moléculaire calculée: 237 aa, 26 kDa
• Poids moléculaire observé: 28 kDa and 30 kDa
• Numéro d’acquisition GenBank: BC012816
• Symbole du gène: TGIF2
• Identification du gène (NCBI): 60436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transforming growth factor-beta-induced factor 2(TGIF2), is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein. This antibody detected two forms of bands of about 28 and 30 kDa, and the upper band appears to result from increased phosphorylation (PMID: 11427533).

Publications

Species	Application	Title
mouse	WB	Cell Rep; Tgif1 Counterbalances the Activity of Core Pluripotency Factors in Mouse Embryonic Stem Cells.; Authors - Bum-Kyu Lee
human	WB	Biochem J; The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome.; Authors - Agata Rozanska
human	WB	Exp Ther Med; Molecular mechanisms in vascular injury induced by hypertension: Expression and role of microRNA-34a.; Authors - Siguan Liu
mouse	WB	Brain Behav; Downregulation of TGIF2 is possibly correlated with neuronal apoptosis and autism-like symptoms in mice.; Authors - Jing Lei