Anticorps Polyclonal de lapin anti-TGM1

TGM1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 12912-3-AP

Synonymes

Epidermal TGase, ICR2, KTG, LI, LI1, TG(K), TGASE, TGase 1, TGase K, TGK, TGM1, Transglutaminase 1, Transglutaminase K

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Galerie de données de validation

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  WB analysis using 12912-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12912-3-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12912-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12912-3-AP

  IP Result of anti-TGM1 (IP:12912-3-AP, 4ug; Detection:12912-3-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human cervix using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: salive humain, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu cutané humain, tissu de col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below

Informations sur le produit

12912-3-AP cible TGM1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TGM1 Protéine recombinante Ag3905
• Nom complet: transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
• Masse moléculaire calculée: 817 aa, 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC034699
• Symbole du gène: TGM1
• Identification du gène (NCBI): 7051
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transglutaminase 1 is an epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelopes in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	J Invest Dermatol; Investigation of Omega-3 Polyunsaturated Fatty Acid Biological Activity in a Tissue-Engineered Skin Model Involving Psoriatic Cells.; Authors - Mélissa Simard
human	WB	J Invest Dermatol; Cathelicidin LL-37 Activates Human Keratinocyte Autophagy through the P2X₇, Mechanistic Target of Rapamycin, and MAPK Pathways; Authors - Risa Ikutama
human	IF	Int J Mol Sci; Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model.; Authors - Andréa Tremblay
mouse	WB,ELISA	Cells; Increased Osteoclastogenesis in Absence of TG2 Is Reversed by Transglutaminase Inhibition-Evidence for the Role for TG1 in Osteoclast Formation; Authors - Sahar Ebrahimi Samani
human	WB	Front Endocrinol (Lausanne); c-Myc Upregulated by High Glucose Inhibits HaCaT Differentiation by S100A6 Transcriptional Activation.; Authors - Jie Zhang