- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-TGM2
TGM2 Polyclonal Antibody for WB, IHC, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (2)
Applications
WB, IHC, IF, IP, ChIP, ELISA
Conjugaison
Non conjugué
N° de cat : 15100-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HUVEC, cellules A549, cellules BxPC-3, cellules HeLa, cellules HepG2, cellules K-562 |
Résultats positifs en IP | cellules HeLa |
Résultats positifs en IHC | tissu de cancer du foie humain, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 7 publications below |
WB | See 26 publications below |
IHC | See 10 publications below |
IF | See 10 publications below |
IP | See 1 publications below |
ChIP | See 1 publications below |
Informations sur le produit
15100-1-AP cible TGM2 dans les applications de WB, IHC, IF, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | canin, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | TGM2 Protéine recombinante Ag7439 |
Nom complet | transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) |
Masse moléculaire calculée | 77 kDa |
Poids moléculaire observé | 77-80 kDa |
Numéro d’acquisition GenBank | BC003551 |
Symbole du gène | TGM2 |
Identification du gène (NCBI) | 7052 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Transglutaminase 2 (TGM2) is a ubiquitous and multifunctional calcium-dependent enzyme belonging to the transglutaminase family. It is best known for its canonical activity of catalyzing the cross-linking of proteins by forming stable ε-(γ-glutamyl)lysine isopeptide bonds, which contributes to extracellular matrix stabilization and wound healing. Beyond this, TGM2 exhibits GTPase activity, allowing it to function as a signaling G-protein in intracellular processes. It is implicated in a wide range of physiological functions, including cell adhesion, proliferation, and apoptosis, as well as pathological conditions such as celiac disease, fibrosis, neurodegenerative disorders, and cancer metastasis, where its dysregulated expression often contributes to disease progression.
Protocole
Product Specific Protocols | |
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WB protocol for TGM2 antibody 15100-1-AP | Download protocol |
IHC protocol for TGM2 antibody 15100-1-AP | Download protocol |
IP protocol for TGM2 antibody 15100-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Nat Commun Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. | ||
Cell Oncol (Dordr) Transglutaminases are oncogenic biomarkers in human cancers and therapeutic targeting of TGM2 blocks chemoresistance and macrophage infiltration in pancreatic cancer
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Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Jon (Verified Customer) (02-15-2022) | DILuted as suggested in %5 milk TBST blocking buffer. We have had problems with other TGM2 antibodies from other companies, but this one seemed no work better and yield a better signal. However, the apparent molecular weight of the TGM2 band appeared to be at a much lower MW than it is supposed to.
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