Anticorps Polyclonal de lapin anti-THADA

• Phare
• Validé par KD/KO

THADA Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12909-1-AP

Synonymes

GITA, KIAA1767, THADA, thyroid adenoma associated

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Galerie de données de validation

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  WB analysis of A549 using 12909-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of CHO cells using 12909-1-AP

  WB result of 12909-1-AP from Takashi Kobayashi.

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  WB analysis of HeLa using 12909-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12909-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse ovary using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse ovary using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse ovary using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse ovary using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat ovary using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat ovary using 12909-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules CHO
• Résultats positifs en IHC: tissu pancréatique humain, tissu ovarien de rat, tissu ovarien de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12909-1-AP cible THADA dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: THADA Protéine recombinante Ag3881
• Nom complet: thyroid adenoma associated
• Masse moléculaire calculée: 220 kDa
• Poids moléculaire observé: 183 kDa
• Numéro d’acquisition GenBank: BC025773
• Symbole du gène: THADA
• Identification du gène (NCBI): 63892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,IP	J Immunother Cancer; THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.; Authors - Chushu Li ; KD Validated