Anticorps Polyclonal de lapin anti-THOC1

THOC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 10920-1-AP

Synonymes

HPR1, hTREX84, Nuclear matrix protein p84, P84, P84N5, THO complex 1, THO complex subunit 1, Tho1, THOC1

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Galerie de données de validation

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  WB analysis of HEK-293 using 10920-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10920-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10920-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10920-1-AP

  IP result of anti-THOC1 (IP:10920-1-AP, 4ug; Detection:10920-1-AP 1:500) with HEK-293 cells lysate 1600 ug.

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  IHC staining of human colon cancer using 10920-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10920-1-AP (THOC1 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 10920-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC1 antibody (10920-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10920-1-AP cible THOC1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: THOC1 Protéine recombinante Ag1352
• Nom complet: THO complex 1
• Masse moléculaire calculée: 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC010381
• Symbole du gène: THOC1
• Identification du gène (NCBI): 9984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in promoted cancer cell apoptosis and reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy. This antibody recognizes the 84kd THOC1 protein.

Publications

Species	Application	Title
human	WB	J Med Virol; SHFL inhibits enterovirus A71 infection by triggering degradation of viral 3Dpol protein via the ubiquitin-proteasome pathway; Authors - Chahui Tan
human	WB	Cancer Lett; TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.; Authors - Sai-Lan Liu
mouse	WB	Elife; Post-transcriptional repression of circadian component CLOCK regulates cancer-stemness in murine breast cancer cells.; Authors - Takashi Ogino
human	WB,IF	Cancers (Basel); Cytoplasmic Clusterin Suppresses Lung Cancer Metastasis by Inhibiting the ROCK1-ERK Axis.; Authors - Shaobo Huang
	WB	Biochem Pharmacol; Modulation of cell physiology by bispecific nanobodies enabling changes in the intracellular localization of organelle proteins; Authors - Akito Tsuruta
human	WB	Cell Oncol (Dordr); Bioactive peptide inhibits acute myeloid leukemia cell proliferation by downregulating ALKBH5-mediated m6A demethylation of EIF4EBP1 and MLST8 mRNA.; Authors - Le Zhang