Anticorps Polyclonal de lapin anti-TIM22

• Phare
• Validé par KD/KO

TIM22 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(33)

Avis (5)

N° de cat : 14927-1-AP

Synonymes

Testis expressed sequence 4, TEX4, TIM22, TIMM22

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Galerie de données de validation

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  WB analysis using 14927-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14927-1-AP (TIM22 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14927-1-AP

  IP result of anti-TIM22 (IP:14927-1-AP, 4ug; Detection:14927-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 14927-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14927-1-AP (TIM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14927-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14927-1-AP (TIM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 31 publications below

Informations sur le produit

14927-1-AP cible TIM22 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIM22 Protéine recombinante Ag6689
• Nom complet: translocase of inner mitochondrial membrane 22 homolog (yeast)
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC002324
• Symbole du gène: TIM22
• Identification du gène (NCBI): 29928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIM22 is a translocase localized in mitochondrial inner membrane. It is implicated in import of nuclear-encoded mitochondrial preproteins to the mitochondria. TIM22 is the essential core of protein insertion complex (TIM22 complex) that consists of TIM22, TIM54, TIM18, and TIM12.

Publications

Species	Application	Title
mouse	WB	Nature; The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.; Authors - Atsushi Hoshino
human	WB	Nat Med; The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.; Authors - Wenzhang Wang ; KD Validated
human	WB	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh
mouse	WB	Cell Metab; Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells.; Authors - Zhilin Hu
human	WB	Cell Metab; Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.; Authors - Marcel Morgenstern
human	WB	Mol Cell; Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.; Authors - Milena Vukotic

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emilie (Verified Customer) (09-24-2025)

I tested it in Western blot (1:1000) and IF (1:200), and it performed well.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: IPSCs derived motoneurons

FH

Manon (Verified Customer) (09-23-2025)

Good mitochondria inner membrane staining by IF

• Applications: Immunofluorescence
• Cell Tissue Type: iPSC-derived motor neurons

FH

Wenhui (Verified Customer) (01-02-2025)

This antibody is sensitive and can give us good clear signal for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cardiac tissues and cardiomyocytes

FH

Wenhui (Verified Customer) (01-02-2025)

This antibody has been working great for us. we can detect bands at correct size for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyocytes.

FH

angela (Verified Customer) (12-27-2024)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: 293

TIM22 Antibody Western Blot validation (1;1000 dilution) in 293 (Cat no:14927-1-AP)

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