Anticorps Polyclonal de lapin anti-TIMM44

• Phare
• Validé par KD/KO

TIMM44 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (1)

N° de cat : 13859-1-AP

Synonymes

MIMT44, TIM44, TIMM44

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Galerie de données de validation

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  WB analysis using 13859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13859-1-AP

  WB result of TIMM44 antibody (13859-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIMM44 transfected HeLa cells.

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  WB analysis of Neuro-2a using 13859-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 13859-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13859-1-AP

  IP result of anti-TIMM44 (IP:13859-1-AP, 4ug; Detection:13859-1-AP 1:1000) with HeLa cells lysate 1280 ug.

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  IP experiment of mouse heart using 13859-1-AP

  IP result of anti-TIMM44(IP:13859-1-AP, 4ug; Detection:13859-1-AP 1:1000) with mouse heart tissue lysate 1680 ug.

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  IF Staining of HepG2 using 13859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 13859-1-AP (TIMM44 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 13859-1-AP

  Immunofluorescent analysis of A549 cells, using TIMM44 antibody 13859-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules Jurkat, cellules Neuro-2a
• Résultats positifs en IP: cellules HeLa, tissu cardiaque de souris
• Résultats positifs en IF: cellules HepG2, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IF: See 3 publications below

Informations sur le produit

13859-1-AP cible TIMM44 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIMM44 Protéine recombinante Ag4834
• Nom complet: translocase of inner mitochondrial membrane 44 homolog (yeast)
• Masse moléculaire calculée: 452 aa, 51 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC033628
• Symbole du gène: TIMM44
• Identification du gène (NCBI): 10469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
human	WB	Cell Metab; Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.; Authors - Marcel Morgenstern
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Mol Cell; Balancing of mitochondrial translation through METTL8-mediated m3C modification of mitochondrial tRNAs.; Authors - Eva Schöller
human	WB	Nat Commun; Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy; Authors - Jonas Benjamin Michaelis
human	WB	Nat Commun; LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.; Authors - Chun-Shik Shin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (04-20-2019)

SDS-PAGE:15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature.Lines on WB:1. BioRad Precision Plus Protein standards2 and 3. Lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ST-Hdh mouse striatal cell line

TIMM44 Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:13859-1-AP)