Anticorps Polyclonal de lapin anti-TK1

• Phare
• Validé par KD/KO

TK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 15691-1-AP

Synonymes

thymidine kinase 1, soluble, Thymidine kinase, cytosolic, TK1, TK2

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Galerie de données de validation

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  WB analysis using 15691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15691-1-AP (TK1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15691-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15691-1-AP (TK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15691-1-AP

  IP result of anti-TK1(IP:15691-1-AP, 4ug; Detection:15691-1-AP 1:800) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of U2OS using 15691-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TK1 antibody (15691-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7, cellules SW-1990
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15691-1-AP cible TK1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TK1 Protéine recombinante Ag8249
• Nom complet: thymidine kinase 1, soluble
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC007986
• Symbole du gène: TK1
• Identification du gène (NCBI): 7083
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).

Publications

Species	Application	Title
human,mouse	WB,IHC	Cell Metab; Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners; Authors - Qing Li ; KD Validated
rat	IF	Theranostics; Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist.; Authors - Lijian Zhang
human	WB	Oxid Med Cell Longev; lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.; Authors - Guibin Liang
mouse,human	WB	Cancer Biol Ther; rAAV9-UPII-TK-EGFP can precisely transduce a suicide gene and inhibit the growth of bladder tumors.; Authors - Foyan Lian
mouse	WB	Adv Biol Regul; Fhit and Wwox loss-associated genome instability: A genome caretaker one-two punch.; Authors - Morgan S Schrock
human	WB	J Cell Mol Med; Down-regulation of TFAM increases the sensitivity of tumour cells to radiation via p53/TIGAR signalling pathway.; Authors - Xu Jiang