Anticorps Polyclonal de lapin anti-TLK2

• Phare
• Validé par KD/KO

TLK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 13979-1-AP

Synonymes

TLK 2, Serine/threonine-protein kinase tousled-like 2, PKU-alpha, PKU ALPHA, HsHPK

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Galerie de données de validation

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  WB analysis of MCF-7 using 13979-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13979-1-AP (TLK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13979-1-AP

  WB result of TLK2 antibody (13979-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLK2 transfected HeLa cells.

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  WB analysis of HeLa using 13979-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13979-1-AP (TLK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13979-1-AP (TLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13979-1-AP (TLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13979-1-AP (TLK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13979-1-AP cible TLK2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TLK2 Protéine recombinante Ag5022
• Nom complet: tousled-like kinase 2
• Masse moléculaire calculée: 772 aa, 88 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC044925
• Symbole du gène: TLK2
• Identification du gène (NCBI): 11011
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tousled-like kinases (TLKs) are a family of serine/threonine kinases located in the nucleus that promote S-phase chromatin assembly and mitotic chromosome segregation. There are two genes in the TLK family-TLK1 and TLK2 (PMID: 14561777). Mutation in TLK2 are associated with an elevated risk of breast cancer (PMID: 27489360). Tousled-like kinase 2 (TLK2) is a key factor in glioblastoma that modulates SRC signaling, thereby driving tumor malignancy. TLK2 is commonly upregulated in glioblastoma, and such upregulation was associated with poor patient outcomes. TLK2 overexpression induced cell growth, migration, invasion, and epithelialmesenchymal transition, and cell cycle arrest, while TLK2 knockdown had the opposite effect (PMID: 30207834). It can detect 3 isoforms of 88, 85 and 82 kDa.

Publications

Species	Application	Title
human	WB	Cancer Gene Ther; Targeting TLK2 inhibits the progression of gastric cancer by reprogramming amino acid metabolism through the mTOR/ASNS axis; Authors - Mingliang Wang ; KD Validated
human	IHC	Front Genet; Overexpression of tousled-like kinase 2 predicts poor prognosis in HBV-related hepatocellular carcinoma patients after radical resection; Authors - Bang Liu
human	WB	Transl Cancer Res; TLK2 promotes progression of hepatocellular carcinoma through Wnt/β-catenin signaling; Authors - Ting He
human,mouse	WB,IP,IF,CoIP	Nat Commun; A TLK2-mediated calcium-driven cell death pathway links neuronal degeneration to nuclear envelope disruption; Authors - Yajie Li ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

A selective and sensitiv antibody. KD validated

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T and HCT116

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