Anticorps Polyclonal de lapin anti-TLR4

• Phare
• Validé par KD/KO

TLR4 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, Microtiter plate binding assay, IP, IHC, IF, CoIP, ChIP, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

422

Avis (1)

N° de cat : 19811-1-AP

Synonymes

ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4

---

Galerie de données de validation

• 

  WB analysis of mouse liver using 19811-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP (TLR4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19811-1-AP (TLR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19811-1-AP (TLR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19811-1-AP (TLR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19811-1-AP (TLR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris,
• Résultats positifs en IHC: tissu placentaire humain, tissu cérébral de souris, tissu de cancer du foie humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 383 publications below
• IHC: See 35 publications below
• IF: See 44 publications below
• IP: See 7 publications below
• ELISA: See 2 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

19811-1-AP cible TLR4 dans les applications de WB, Microtiter plate binding assay, IP, IHC, IF, CoIP, ChIP, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, Lapin, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TLR4 Protéine recombinante Ag13841
• Nom complet: toll-like receptor 4
• Masse moléculaire calculée: 839 aa, 96 kDa
• Poids moléculaire observé: 90-110 kDa
• Numéro d’acquisition GenBank: BC117422
• Symbole du gène: TLR4
• Identification du gène (NCBI): 7099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TLR4, also named CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described. The calculated molecular weights of the three TLR4 isoforms are 96, 91, and 73 kDa.

Publications

Species	Application	Title
mouse	WB	Adv Mater; Biomimetic Immunosuppressive Exosomes that Inhibit Cytokine Storms Contribute to the Alleviation of Sepsis.; Authors - Yinan Li
human	ELISA	J Clin Invest; Longistatin in tick saliva blocks advanced glycation end-product receptor activation.; Authors - Takeshi Anisuzzaman
human	WB	Gut Microbes; Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway; Authors - Jian-Wei Zhang
mouse	IF,WB	Nat Commun; TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection.; Authors - Jing Geng
mouse	WB	Nat Commun; Alarmin-painted exosomes elicit persistent antitumor immunity in large established tumors in mice.; Authors - Bingfeng Zuo ; KO Validated
mouse	IP	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Sara (Verified Customer) (10-25-2020)

B cells (mouse) were lysated in RIPA buffer with protein and phosphatase inhibitors. 10 µg of protein per lane were loaded in a 10% PAGE-SDS gel. Proteins were transferred to a PVFD membrane (Turbo Blot system) and incubated overnight with 1:1000 of anti-TLR4, followed by anti-rabbit-HRP (1:10.000) on the next day. Two bands of around 70 kDa are detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary B cells from mouse spleen

TLR4 Antibody Western Blot validation (1:1000 dilution) in Primary B cells from mouse spleen (Cat no:19811-1-AP)

Produits associés

CD14 Polyclonal antibody

17000-1-AP

TLR4 Monoclonal antibody

66350-1-Ig

CD14 Monoclonal antibody

60253-1-Ig

LY96/MD2 Polyclonal antibody

11784-1-AP

NF-κB p65 Polyclonal antibody

10745-1-AP

TLR6 Polyclonal antibody

22240-1-AP

CD36 Polyclonal antibody

18836-1-AP

NOD2 Polyclonal antibody

20980-1-AP