Anticorps Polyclonal de lapin anti-TLR6

• Phare
• Validé par KD/KO

TLR6 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 22240-1-AP

Synonymes

CD286, toll like receptor 6, Toll-like receptor 6

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 22240-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22240-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22240-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 22240-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 22240-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 22240-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22240-1-AP (TLR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules HT-1080, cellules Jurkat, tissu cérébral humain, tissu de thymus de rat, tissu de thymus de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below

Informations sur le produit

22240-1-AP cible TLR6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TLR6 Protéine recombinante Ag17686
• Nom complet: toll-like receptor 6
• Masse moléculaire calculée: 480 aa, 56 kDa
• Poids moléculaire observé: 95-110 kDa
• Numéro d’acquisition GenBank: BC111755
• Symbole du gène: TLR6
• Identification du gène (NCBI): 10333
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TLR6, also known as CD286, belongs to the Toll-like receptor (TLR) family, which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR6 can form a heterodimer with TLR2. TLR6 and TLR2 are recruited to the macrophage phagosome, where they recognize peptidoglycan, a Gram-positive pathogen component (PMID: 11095740). TLR6 interacts with CD36, following CD36 stimulation by oxLDL or amyloid-beta 42, and forms a heterodimer with TLR4. The trimeric complex is internalized and triggers an inflammatory response.

Publications

Species	Application	Title
mouse	WB	ACS Nano; Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment.; Authors - Han Lin
mouse,human	WB	BMC Med; HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis.; Authors - Xiaoyu Xie ; KD Validated
human	WB	J Med Chem; Design, Synthesis, and Structure-Activity Relationship of N-Aryl-N'-(thiophen-2-yl)thiourea Derivatives as Novel and Specific Human TLR1/2 Agonists for Potential Cancer Immunotherapy.; Authors - Zhipeng Chen
human		Bioorg Chem; Synthesis, evaluation and molecular dynamics study of human toll-like receptor 2/6 specific monoacyl lipopeptides as candidate immunostimulants; Authors - Yueyue Zhu
rat	WB	J Gerontol A Biol Sci Med Sci; METTL3 promotes nucleus pulposus cell senescence in intervertebral disc degeneration by regulating TLR2 m6A methylation and gut microbiota; Authors - Shuangfei Ni
human	WB	Eur J Med Chem; Structure-activity relationship study and biological evaluation of SAC-Garlic acid conjugates as novel anti-inflammatory agents.; Authors - Jingjie Bi

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