Anticorps Polyclonal de lapin anti-TMED2

TMED2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 11981-1-AP

Synonymes

RNP24, Membrane protein p24A, p24, p24 family protein beta-1, p24beta1

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Galerie de données de validation

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  WB analysis using 11981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11981-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11981-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 11981-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11981-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11981-1-AP (TMED2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 11981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11981-1-AP (TMED2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 11981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11981-1-AP (TMED2 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cutané de souris, tissu hépatique de souris, tissu pancréatique de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11981-1-AP cible TMED2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMED2 Protéine recombinante Ag2607
• Nom complet: transmembrane emp24 domain trafficking protein 2
• Masse moléculaire calculée: 201 aa, 23 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC025957
• Symbole du gène: TMED2
• Identification du gène (NCBI): 10959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transmembrane emp24 domain-containing protein 2 (TMED2) belongs to the p24 receptor family, being mainly involved in vesicular trafficking of proteins between the endoplasmic reticulum and Golgi apparatus.

Publications

Species	Application	Title
human	WB	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li
rat	WB	PLoS Biol; TMED9 coordinates the clearance of misfolded GPI-anchored proteins out of the ER and into the Golgi; Authors - Elsa Ronzier
human	WB	BMC Biol; ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.; Authors - Xihua Yue
human	WB,IF	BMC Cancer; The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.; Authors - Xue Yang
human	WB	Adv Sci (Weinh); TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.; Authors - Hak Park
human	WB	Ann Transl Med; Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer; Authors - Zheng Fang ; KD Validated

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