Anticorps Polyclonal de lapin anti-TMEM106B (150-274aa)

• Phare
• Validé par KD/KO

TMEM106B (150-274aa) Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (2)

N° de cat : 20995-1-AP

Synonymes

TMEM106B, Transmembrane protein 106B

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Galerie de données de validation

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  WB analysis using 20995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20995-1-AP (TMEM106B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20995-1-AP

  WB result of TMEM106B antibody (20995-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM106B transfected HeLa cells.

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  IHC staining of rat brain using 20995-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20995-1-AP (TMEM106B (150-274aa) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 20995-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20995-1-AP (TMEM106B (150-274aa) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu cérébral de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 5 publications below

Informations sur le produit

20995-1-AP cible TMEM106B (150-274aa) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM106B (150-274aa) Protéine recombinante Ag14264
• Nom complet: transmembrane protein 106B
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31-35 kDa, 40-55 kDa
• Numéro d’acquisition GenBank: BC033901
• Symbole du gène: TMEM106B
• Identification du gène (NCBI): 54664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression. TMEM106B can be showed as 31-55 kDa and 70-90 kDa (Glycosylated or Dimer) form in western blot test. (PMID: 27543298, 22895706, PMID: 23136129).

Publications

Species	Application	Title
human		Cell; Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases.; Authors - Andrew Chang
human	WB	J Neuroinflammation; Progranulin haploinsufficiency mediates cytoplasmic TDP-43 aggregation with lysosomal abnormalities in human microglia; Authors - Wonjae Sung
human	WB	J Neurosci; TMEM106B, the risk gene for frontotemporal dementia, is regulated by the microRNA-132/212 cluster and affects progranulin pathways.; Authors - Chen-Plotkin Alice S AS
human	WB	Alzheimers Res Ther; TMEM106B expression is reduced in Alzheimer's disease brains.; Authors - Jun-Ichi Satoh
mouse	IF	Mol Brain; TMEM106B, a frontotemporal lobar dementia (FTLD) modifier, associates with FTD-3-linked CHMP2B, a complex of ESCRT-III.; Authors - Mi-Hee Jun
human	WB	PLoS One; Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.; Authors - Kui Xiao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Q (Verified Customer) (05-08-2025)

It seems in WB, it works better with human sample than with mouse sample.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain lysate, human postmortem brain lysate

FH

Carolyn (Verified Customer) (02-18-2022)

good signal, low background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human brain tissue extract

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