Anticorps Monoclonal anti-TMEM106B

• Phare
• Validé par KD/KO

TMEM106B Monoclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

5D1F8

N° de cat : 60333-1-Ig

Synonymes

TMEM106B, transmembrane protein 106B

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Galerie de données de validation

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  WB analysis using 60333-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60333-1-Ig

  WB result of TMEM106B antibody (60333-1-Ig; 1:2000; room temperature for 1.5 hours) with wild-type and TMEM106B knockout HeLa cells.

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  WB analysis of HeLa using 60333-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60333-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60333-1-Ig

  IP result of anti-TMEM106B(IP:60333-1-Ig, 5ug; Detection:60333-1-Ig 1:500) with HeLa cells lysate 1720 ug.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

60333-1-Ig cible TMEM106B dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TMEM106B Protéine recombinante Ag21448
• Nom complet: transmembrane protein 106B
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: ~40 kDa
• Numéro d’acquisition GenBank: BC033901
• Symbole du gène: TMEM106B
• Identification du gène (NCBI): 54664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.

Publications

Species	Application	Title
human	IHC	Cell; Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases.; Authors - Andrew Chang
human	WB	Brain; C-terminal TMEM106B fragments in human brain correlate with disease-associated TMEM106B haplotypes; Authors - Cristina T Vicente
human	WB	Pathol Res Pract; TESC acts as a prognostic factor and promotes epithelial-mesenchymal transition progression in esophageal squamous carcinoma; Authors - Yanxin Dong
human	IHC	Acta Neuropathol; Accumulation of TMEM106B C-terminal fragments in neurodegenerative disease and aging; Authors - Jolien Perneel
human	WB	Front Cell Neurosci; Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation; Authors - Claudia S Bauer ; KD Validated