Anticorps Polyclonal de lapin anti-TMEM192

TMEM192 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 28263-1-AP

Synonymes

FLJ38482, TMEM192, transmembrane protein 192

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Galerie de données de validation

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  WB analysis using 28263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28263-1-AP (TMEM192 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain, tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

28263-1-AP cible TMEM192 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM192 Protéine recombinante Ag27576
• Nom complet: transmembrane protein 192
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC036301
• Symbole du gène: TMEM192
• Identification du gène (NCBI): 201931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transmembrane protein 192 (TMEM192) has been previously identified in proteomic analyses of lysosomal membranes.TMEM192 shows a wide expression pattern in mouse tissues. Interestingly, TMEM192 was found to be highly expressed in tumor cell lines, while it was not expressed or detected at low levels in normal cell lines(PMID: 22736246). TMEM192 can be detected as 30 kDa.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; Activation of lysophagy by a TBK1-SCFFBXO3-TMEM192-TAX1BP1 axis in response to lysosomal damage; Authors - Na Yeon Park
human	IHC	Mol Ther; Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase; Authors - Yang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (09-27-2020)

This antibody is very good, and I recommend this to others who are doing lyso-IP using a tagged TMEM192.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

TMEM192 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in HEK293T (Cat no:28263-1-AP)

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