Anticorps Polyclonal de lapin anti-TMEM87A

TMEM87A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 25091-1-AP

Synonymes

Transmembrane protein 87A, TM87A, PSEC0094, Elkin1

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Galerie de données de validation

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  WB analysis using 25091-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 25091-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25091-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 25091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 25091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 25091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 25091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules MDA-MB-453s, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25091-1-AP cible TMEM87A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM87A Protéine recombinante Ag18715
• Nom complet: transmembrane protein 87A
• Masse moléculaire calculée: 555 aa, 63 kDa
• Poids moléculaire observé: 63-70 kDa
• Numéro d’acquisition GenBank: BC005335
• Symbole du gène: TMEM87A
• Identification du gène (NCBI): 25963
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a band around 60 kd, however, this band is not sensitive to deglycosylation. What's worse, this band also occurred in solo loading buffer.

• Applications: Western Blot

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