Anticorps Polyclonal de lapin anti-TMPO/LAP2

• Phare
• Validé par KD/KO

TMPO/LAP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 14651-1-AP

Synonymes

CMD1T, LAP2, LEMD4, PRO0868, Splenin Thymopentin, thymopoietin, Thymopoietin isoform alpha, TMPO, TMPO/LAP2, TP, TP alpha, TP5, TPRP isoform alpha

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Galerie de données de validation

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  WB analysis using 14651-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14651-1-AP

  WB result of TMPO/LAP2 antibody (14651-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMPO/LAP2 transfected HeLa cells.

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  IP experiment of HeLa using 14651-1-AP

  IP Result of anti-LAP2 (IP:14651-1-AP, 4ug; Detection:14651-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human breast cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14651-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (14651-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa cells using 14651-1-AP

  IF result of anti-TMPO (14651-1-AP) in Hela cell by Dr. Ralph Kehlenbach.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du foie humain, tissu hépatique humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

14651-1-AP cible TMPO/LAP2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMPO/LAP2 Protéine recombinante Ag6247
• Nom complet: thymopoietin
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 75 kDa, 51 kDa and 39 kDa
• Numéro d’acquisition GenBank: BC053675
• Symbole du gène: TMPO
• Identification du gène (NCBI): 7112
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).

Publications

Species	Application	Title
mouse		Nat Commun; The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.; Authors - Ying Zhang
human	IF	J Cell Biol; A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies.; Authors - Tino Pleiner
human	WB	Sci Rep; MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.; Authors - Yuhua Fu
bovine	WB	J Dairy Sci; Anti-inflammation of epicatechin mediated by TMEM35A and TMPO in bovine mammary epithelial cell line cells and mouse mammary gland.; Authors - Xiao Ma ; KO Validated
human	WB	World J Surg Oncol; Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells.; Authors - Lin Zhang ; KD Validated
mouse	IF	Theranostics; Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane.; Authors - Xiaolong Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lauren (Verified Customer) (02-20-2020)

Tested fixes include -20'C Methanol for 10 mins, and both 2% and 4% Formaldehyde for 20 mins. We saw a good signal in all three fixes.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mIMCD3

TMPO/LAP2 Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:14651-1-AP)