Anticorps Polyclonal de lapin anti-TMPRSS4

• Phare
• Validé par KD/KO

TMPRSS4 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 11283-1-AP

Synonymes

CAPH2, Channel activating protease 2, MT SP2, TMPRSS3, TMPRSS4

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Galerie de données de validation

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  WB analysis of SW 1990 using 11283-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 11283-1-AP (TMPRSS4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW 1990 using 11283-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 11283-1-AP (TMPRSS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 11283-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 11283-1-AP (TMPRSS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 11283-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11283-1-AP (TMPRSS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11283-1-AP (TMPRSS4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11283-1-AP (TMPRSS4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11283-1-AP (TMPRSS4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11283-1-AP (TMPRSS4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11283-1-AP (TMPRSS4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11283-1-AP (TMPRSS4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11283-1-AP (TMPRSS4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 11283-1-AP (TMPRSS4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 11283-1-AP (TMPRSS4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11283-1-AP (TMPRSS4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11283-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11283-1-AP (TMPRSS4 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules SW-1990, cellules BxPC-3, tissu d'estomac de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 12 publications below
• IF: See 3 publications below

Informations sur le produit

11283-1-AP cible TMPRSS4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMPRSS4 Protéine recombinante Ag1804
• Nom complet: transmembrane protease, serine 4
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC011703
• Symbole du gène: TMPRSS4
• Identification du gène (NCBI): 56649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TMPRSS4, also named as CAPH2 and MT-SP2, belongs to the peptidase S1 family. TMPRSS4 is a novel type II transmembrane serine protease that is highly expressed on the cell surface in pancreatic, thyroid and other cancer tissues. It promotes invasion, migration and metastasis of human tumor cells by facilitating an epithelial-mesenchymal transition(EMT)(PMID:20118200).

Publications

Species	Application	Title
human	WB	Gastroenterology; Angiotensin-converting Enzyme 2-containing Extracellular Vesicles and Exomeres Bind the Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein.; Authors - Qin Zhang
human	WB	Cancer Res; TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation.; Authors - David J Drasin
mouse	WB	Int J Mol Sci; Cardiac and Renal SARS-CoV-2 Viral Entry Protein Regulation by Androgens and Diet: Implications for Polycystic Ovary Syndrome and COVID-19.; Authors - Samar Rezq
human	WB,IHC,IF	Carcinogenesis; TMPRSS4 induces invasion and epithelial-mesenchymal transition through upregulation of integrin alpha5 and its signaling pathways.; Authors - Kim Semi S
human, mouse	WB, IHC, IF	Cancer Lett; TMPRSS4 induces cancer stem cell-like properties in lung cancer cells and correlates with ALDH expression in NSCLC patients.; Authors - Arrate L de Aberasturi
human	IHC	Front Immunol; TSPAN1, TMPRSS4, SDR16C5, and CTSE as Novel Panel for Pancreatic Cancer: A Bioinformatics Analysis and Experiments Validation.; Authors - Hua Ye

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