Anticorps Recombinant de lapin anti-TNF beta

TNF beta Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240393C3

N° de cat : 98056-1-RR

Synonymes

LTA, TNF β, Lymphotoxin-alpha, Lymphotoxin alpha, LT alpha

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Galerie de données de validation

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  FC experiment of human PBMCs using 98056-1-RR

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF beta Rabbit Recombinant Antibody (98056-1-RR, Clone: 240393C3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD3. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98056-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98056-1-RR against Human TNF Beta were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en FC (Intra): Anti-CD3/CD28 treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98056-1-RR cible TNF beta dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: lymphotoxin alpha (TNF superfamily, member 1)
• Masse moléculaire calculée: 205 aa, 22 kDa
• Numéro d’acquisition GenBank: BC034729
• Symbole du gène: TNF beta
• Identification du gène (NCBI): 4049
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

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