Anticorps Recombinant de lapin anti-TNF-alpha

TNF-alpha Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

240668B11

N° de cat : FITC-98064

Synonymes

TNF, TNF alpha, TNF-a, 240668B11, C-domain 1



Applications testées

Résultats positifs en FC (Intra)PMA, Ionomycin and Brefeldin A treated human PBMCs,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-98064 cible TNF-alpha dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Protéine recombinante
Nom complet tumor necrosis factor (TNF superfamily, member 2)
Masse moléculaire calculée26 kDa
Numéro d’acquisition GenBankNM_000594.4
Symbole du gène TNF-alpha
Identification du gène (NCBI) 7124
Conjugaison FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

Protocole

Product Specific Protocols
FC protocol for FITC Plus TNF-alpha antibody FITC-98064Download protocol
Standard Protocols
Click here to view our Standard Protocols
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