Anticorps Recombinant de lapin anti-TNF beta

TNF beta Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240393C3

N° de cat : CL647-98056

Synonymes

LTA, 240393C3, LT alpha, Lymphotoxin alpha, Lymphotoxin-alpha

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Galerie de données de validation

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  FC experiment of human PBMCs using CL647-98056

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human TNF beta Rabbit RecAb (CL647-98056, Clone:240393C3). Cells were co-stained with FITC Plus Anti-Human CD3 (OKT3) (FITC-65133, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): Anti-CD3/CD28 treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98056 cible TNF beta dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TNF beta Protéine recombinante Eg0836
• Nom complet: lymphotoxin alpha (TNF superfamily, member 1)
• Masse moléculaire calculée: 205 aa, 22 kDa
• Numéro d’acquisition GenBank: BC034729
• Symbole du gène: TNF beta
• Identification du gène (NCBI): 4049
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

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