Anticorps Monoclonal anti-TNFAIP3

TNFAIP3 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3A11G6

N° de cat : CL594-66695

Synonymes

OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37

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Galerie de données de validation

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  IF Staining of HeLa using CL594-66695

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated TNFAIP3 antibody (CL594-66695, Clone: 3A11G6) at dilution of 1:200, F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).

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  FC experiment of HeLa using CL594-66695

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TNFAIP3 (CL594-66695, Clone:3A11G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66695 cible TNFAIP3 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TNFAIP3 Protéine recombinante Ag18150
• Nom complet: tumor necrosis factor, alpha-induced protein 3
• Masse moléculaire calculée: 790 aa, 90 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC114480
• Symbole du gène: TNFAIP3
• Identification du gène (NCBI): 7128
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and is involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

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