Anticorps Polyclonal de lapin anti-TNIP1

• Phare
• Validé par KD/KO

TNIP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 15104-1-AP

Synonymes

ABIN 1, ABIN1, ABIN-1, HIV 1 Nef interacting protein, hVAN, KIAA0113, NAF1, Nef associated factor 1, Nip40 1, TNFAIP3 interacting protein 1, TNIP1, VAN

---

Galerie de données de validation

• 

  WB analysis using 15104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15104-1-AP (TNIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15104-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15104-1-AP (TNIP1 Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15104-1-AP

  IP result of anti-TNIP1(IP:15104-1-AP, 4ug; Detection:15104-1-AP 1:6000) with HeLa cells lysate 1320 ug.

• 

  IHC staining of human lymphoma using 15104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15104-1-AP (TNIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 15104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15104-1-AP (TNIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15104-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15104-1-AP (TNIP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules U2OS, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

15104-1-AP cible TNIP1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNIP1 Protéine recombinante Ag1561
• Nom complet: TNFAIP3 interacting protein 1
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC014008
• Symbole du gène: TNIP1
• Identification du gène (NCBI): 10318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNIP1, also named as KIAA0113, NAF1, Nip40-1, VAN and ABIN-1, is involved in inhibition of nuclear factor- B (NF- B) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3). TNIP1 repression of RARs in the presence of RA places it in a small category of corepressors of agonist-bound NRs.

Publications

Species	Application	Title
human	IF	Mol Cell; TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1; Authors - François Le Guerroué
mouse	WB	Cell Death Differ; Ubiquitin-binding domain in ABIN1 is critical for regulating cell death and inflammation during development.; Authors - Ming Li
human	WB	BMC Biol; A functional genomics screen for microRNA regulators of NF-kappaB signaling.; Authors - Olarerin-George Anthony O AO ; KD Validated
human	WB,IF	Retrovirology; Immune regulator ABIN1 suppresses HIV-1 transcription by negatively regulating the ubiquitination of Tat.; Authors - Shiyou Chen ; KD Validated
human	WB	J Cell Biochem; ABIN1 inhibits HDAC1 ubiquitination and protects HDAC1 from both proteasome- and lysozyme-dependent degradation.; Authors - Yuhong Ma ; KO Validated
mouse	WB	Clin Exp Pharmacol Physiol; ABIN-1 protects chondrocytes from lipopolysaccharide-induced inflammatory injury through the inactivation of NF-κB signalling.; Authors - Kan Peng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Heiko (Verified Customer) (09-04-2019)

PVDF membrane blocked in 5% milk TBST 1 h at RT.Primary antibody incubation overnight 4°C.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

TNIP1 Antibody Western Blot, validation (1:1000 dilution) in HeLa cells (Cat no:15104-1-AP)