Anticorps Polyclonal de lapin anti-Cardiac Troponin T

Cardiac Troponin T Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(77)

Avis (2)

N° de cat : 15513-1-AP

Synonymes

cTnT, TNNT2, Troponin T, cardiac muscle

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Galerie de données de validation

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  WB analysis using 15513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15513-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (TNNT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15513-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 15513-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15513-1-AP (Cardiac Troponin T antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 15513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15513-1-AP (Cardiac Troponin T antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 15513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin T antibody (15513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 15513-1-AP

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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  IF Staining of mouse heart using 15513-1-AP

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en IF-Fro: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IHC: See 5 publications below
• IF: See 52 publications below

Informations sur le produit

15513-1-AP cible Cardiac Troponin T dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cardiac Troponin T Protéine recombinante Ag7716
• Nom complet: troponin T type 2 (cardiac)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34-40 kDa
• Numéro d’acquisition GenBank: BC002653
• Symbole du gène: Cardiac Troponin T
• Identification du gène (NCBI): 7139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.

Publications

Species	Application	Title
mouse	IF	Nature; Brahma safeguards canalization of cardiac mesoderm differentiation.; Authors - Swetansu K Hota
pig	IF	Cell Stem Cell; Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation; Authors - Jiaowei Wang
human	IF	Sci Transl Med; Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy; Authors - Takahiko Nishiyama
human	IF	ACS Nano; Intrinsic Color Sensing System Allows for Real-Time Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.; Authors - Yiqi Gong
mouse	IF	Acta Pharm Sin B; Hippo pathway-manipulating neutrophil-mimic hybrid nanoparticles for cardiac ischemic injury via modulation of local immunity and cardiac regeneration; Authors - Qiaozi Wang
mouse	IF	Biomaterials; Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure; Authors - Lingjun Tong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rebecca (Verified Customer) (03-16-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPS-CM

Cardiac Troponin T Antibody Western Blot validation (1:1000 dilution) in iPS-CM (Cat no:15513-1-AP)

FH

Lianjie (Verified Customer) (07-26-2019)

This antibody works well.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse embryonic heart

Cardiac Troponin T Antibody Immunohistochemistry, validation (1:50 dilution) in Mouse embryonic heart (Cat no:15513-1-AP)

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