Anticorps Polyclonal de lapin anti-TOB1

• Phare
• Validé par KD/KO

TOB1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 14915-1-AP

Synonymes

Transducer of erbB-2 1, Transducer of erbB 2 1, TOB, Protein Tob1, PIG49

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Galerie de données de validation

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  WB analysis of HeLa using 14915-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14915-1-AP (TOB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14915-1-AP

  IP result of anti-TOB1 (IP:14915-1-AP, 4ug; Detection:14915-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human stomach cancer using 14915-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14915-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14915-1-AP cible TOB1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TOB1 Protéine recombinante Ag6714
• Nom complet: transducer of ERBB2, 1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38-45 kDa
• Numéro d’acquisition GenBank: BC031406
• Symbole du gène: TOB1
• Identification du gène (NCBI): 10140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.

Publications

Species	Application	Title
human	WB	Oncotarget; Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.; Authors - Jin Zhu
mouse	IHC	RNA Biol; NEDD4 triggers FOXA1 ubiquitination and promotes colon cancer progression under microRNA-340-5p suppression and ATF1 upregulation.; Authors - Meng Yue
human	WB	RNA Biol; Preferential translation of p53 target genes.; Authors - Miharu Hisaoka
human	WB	Am J Transl Res; Phosphorylation of TOB1 at T172 and S320 is critical for gastric cancer proliferation and progression.; Authors - Dong Wang
human	WB,IHC	Cancer Med; TOB1 suppresses proliferation in K-Ras wild-type pancreatic cancer.; Authors - Yuru Bai
mouse,human	WB,IF,IP	Commun Biol; TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression; Authors - Zhongxia Yu ; KO Validated

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