Anticorps Polyclonal de lapin anti-TOE1

• Phare
• Validé par KD/KO

TOE1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 16203-1-AP

Synonymes

Target of EGR1 protein 1, TOE1

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Galerie de données de validation

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  WB analysis using 16203-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16203-1-AP (TOE1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16203-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16203-1-AP (TOE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 16203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16203-1-AP (TOE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16203-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16203-1-AP (TOE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16203-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16203-1-AP (TOE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16203-1-AP (TOE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16203-1-AP

  Immunofluorescent analysis of HepG2 cells, using TOE1 antibody 16203-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, tissu de thymus de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

16203-1-AP cible TOE1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TOE1 Protéine recombinante Ag9172
• Nom complet: target of EGR1, member 1 (nuclear)
• Masse moléculaire calculée: 510 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC009364
• Symbole du gène: TOE1
• Identification du gène (NCBI): 114034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

Publications

Species	Application	Title
mouse	IF	Theriogenology; Knockdown of Toe1 causes developmental arrest during the morula-to-blastocyst transition in mice; Authors - Hongcheng Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rhys (Verified Customer) (08-26-2019)

Superb antibody that works well for the detection for endogenous human TOE1 protein by western blotting.Lane 1: K562 wtGapLane 2: K562 control (empty vector)Lane 3: K562 TOE1 overexpressionGapLane 4: K562 scrambled shRNA controlLane 5: K562 TOE1 shRNA #1Lane 6: K562 TOE1 shRNA #2Lane 7: K562 TOE1 shRNA #3Lane 8: K562 TOE1 shRNA #4Lane 9: K562 TOE1 shRNA #5

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: K562 cells (Chronic Myeloid Leukaemia)

TOE1 Antibody Western Blot, validation (1/1000 dilution) in K562 cells (Chronic Myeloid Leukaemia) (Cat no:16203-1-AP)