Anticorps Monoclonal anti-TOM20

TOM20 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(71)

CloneNo.

1D6F5

Avis (4)

N° de cat : 66777-1-Ig

Synonymes

TOMM20, TOMM 20, tom 20, MOM19, MAS20

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Galerie de données de validation

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  WB analysis using 66777-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66777-1-Ig (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66777-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66777-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66777-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66777-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66777-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (66777-1-Ig, Clone: 1D6F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 66777-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (66777-1-Ig, Clone: 1D6F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

• 

  FC experiment of HeLa using 66777-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TOM20 (66777-1-Ig, Clone:1D6F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 30 publications below
• IHC: See 2 publications below
• IF: See 52 publications below

Informations sur le produit

66777-1-Ig cible TOM20 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain, poulet, singe
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TOM20 Protéine recombinante Ag2378
• Nom complet: translocase of outer mitochondrial membrane 20 homolog (yeast)
• Masse moléculaire calculée: 145 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC000882
• Symbole du gène: TOM20
• Identification du gène (NCBI): 9804
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

Publications

Species	Application	Title
human	WB	Science; Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.; Authors - Qiang Wang
human	IF	J Pineal Res; Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics; Authors - Ye Young Shin
human		Brain; Inhibiting mitochondrial fission rescues degeneration in hereditary spastic paraplegia neurons.; Authors - Zhenyu Chen
	WB	Redox Biol; Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.; Authors - Wei Ou
human	IF	Br J Cancer; Loss of NDUFS1 promotes gastric cancer progression by activating the mitochondrial ROS-HIF1α-FBLN5 signaling pathway; Authors - Tao Chen
bovine	IF	Phytomedicine; Forsythiaside A attenuates mastitis via PINK1/Parkin-mediated mitophagy; Authors - Jingjing Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Pierre (Verified Customer) (09-26-2025)

Very Good

• Applications: Western Blot
• Cell Tissue Type: T cells

FH

Paula (Verified Customer) (08-06-2024)

Worked well for human bone marrow mesenchymal stem cells. Secondary antibody dilution used 1:400 for 90min room temp.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human

TOM20 Antibody Immunofluorescence validation (1:500 dilution) in Human (Cat no:66777-1-Ig)

FH

Elena (Verified Customer) (05-06-2024)

Specific detection

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: iPSC-derived motor neurons

FH

Boyan (Verified Customer) (02-22-2024)

good for imaging experiments

• Applications: Immunofluorescence

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