Anticorps Polyclonal de lapin anti-TOMM40

• Phare
• Validé par KD/KO

TOMM40 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

78

Avis (4)

N° de cat : 18409-1-AP

Synonymes

C19orf1, D19S1177E, p38.5, PER EC1, PEREC1, Protein Haymaker, TOM40, TOMM40

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Galerie de données de validation

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  WB analysis using 18409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18409-1-AP (TOMM40 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18409-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18409-1-AP (TOM40 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 18409-1-AP

  IP Result of anti-TOM40 (IP:18409-1-AP, 4ug; Detection:18409-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18409-1-AP (TOM40 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 18409-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18409-1-AP (TOM40 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 18409-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOMM40 antibody (18409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

• 

  IF Staining of HeLa using 18409-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  FC experiment of HepG2 using 18409-1-AP

  1X10^6 HepG2 cells were stained with .2ug TOM40 antibody (18409-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, tissu cérébral humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque de souris, tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 66 publications below
• IHC: See 1 publications below
• IF: See 15 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

18409-1-AP cible TOMM40 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TOMM40 Protéine recombinante Ag13065
• Nom complet: translocase of outer mitochondrial membrane 40 homolog (yeast)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC017224
• Symbole du gène: TOMM40
• Identification du gène (NCBI): 10452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.

Publications

Species	Application	Title
mouse	WB	Nature; The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.; Authors - Atsushi Hoshino
human	WB	Nature; In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.; Authors - Milica Momcilovic
human	IF	Cell; Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.; Authors - Tara E Tracy
human	WB	Cell Discov; The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth; Authors - Xiaojun Ren
human	WB	Cell Metab; Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.; Authors - Marcel Morgenstern ; KD Validated
monkey	WB,IF	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nuo (Verified Customer) (01-28-2023)

Good antibody for WB with high titer.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

TOMM40 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:18409-1-AP)

FH

Süleyman (Verified Customer) (01-23-2022)

TOMM40 antibody (Green) is used 1:100 dilution (2 hour at RT) and 1:700 of Alexa Fluor 488 secondary antibody (1 hour RT). 4% Paraformaldehyde fixation for 10 min at RT. Permeabilization with 0.3% Triton X for 10-15 minutes at RT. 5% FBS in PBS used for blocking for 1 hour at RT. The image is colocalized with TOMM20 (Red). Blue is DAPI. As you can see there is colocalization with TOMM20 with TOMM40. It is a perfect colocalization. You might also reduce concentration to 1:300 as it was used low power of laser.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa FlipIn

TOMM40 Antibody Immunofluorescence validation (1:100 dilution) in HeLa FlipIn (Cat no:18409-1-AP)

FH

Tom (Verified Customer) (03-23-2021)

Cos7 cells fixed in 4% PFA and permeabilized in 0.1% Triton X-100. Cells blocked in 1% BSA in PBS.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Cos7

TOMM40 Antibody Immunofluorescence validation (1:200 dilution) in Cos7 (Cat no:18409-1-AP)

FH

Daniela (Verified Customer) (01-08-2020)

Immunoblot of total cell lysate of HeLa cell gives three bands, one specific for human Tom40 above 37 KDa on 15% acrylamide gel, the other two no specific and with much lower intensity. The anti-Tom40 antibody worked very well and specifically when conjugated with magnetic beads for immunoprecipitation.

• Applications: Western Blot, Immunoprecipitation, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

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