Anticorps Polyclonal de lapin anti-TOP2A

• Phare
• Validé par KD/KO

TOP2A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 24641-1-AP

Synonymes

DNA topoisomerase 2 alpha, TOP II, TOP2, TOP2A, TOPII, TP2A

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Galerie de données de validation

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  WB analysis of HepG2 using 24641-1-AP

  WB result of TOP2A antibody (24641-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells.

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  WB analysis using 24641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24641-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24641-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24641-1-AP

  IP Result of anti-TOP2A (IP:24641-1-AP, 4ug; Detection:24641-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human pancreas cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

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  IF Staining of A431 using 24641-1-AP

  Immunofluorescent analysis of A431 cells using 24641-1-AP (TOP2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of A431 using 24641-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (24641-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules MDA-MB-231
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

24641-1-AP cible TOP2A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TOP2A Protéine recombinante Ag19551
• Nom complet: topoisomerase (DNA) II alpha 170kDa
• Masse moléculaire calculée: 1531 aa, 174 kDa
• Poids moléculaire observé: 174 kDa
• Numéro d’acquisition GenBank: BC140791
• Symbole du gène: TOP2A
• Identification du gène (NCBI): 7153
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol; Chemotherapy- and Immune-Related Gene Panel in Prognosis Prediction and Immune Microenvironment of SCLC.; Authors - Meng-Yu Chen
human	WB,IF,IP	Mol Biol Cell; SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.; Authors - Ryuta Watanabe
		Dev Biol; Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes.; Authors - Jacinta H Martin
human	WB	Chem Biol Interact; SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.; Authors - Jingjun Wu
		Oncol Rep; Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study.; Authors - Jun-Song Liu
human	IHC	J Clin Neurosci; High prevalence of developmental venous anomaly in adult patients with midline thalamic diffuse gliomas.; Authors - Xingwang Zhou