Anticorps Polyclonal de lapin anti-TOP2B

• Phare
• Validé par KD/KO

TOP2B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 20549-1-AP

Synonymes

DNA topoisomerase 2 beta, TOP2B, top2beta, TOPIIB

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Galerie de données de validation

• 

  WB analysis of HeLa using 20549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20549-1-AP

  WB result of TOP2B antibody (20549-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells.

• 

  WB analysis of HeLa using 20549-1-AP

  WB result of TOP2B antibody (20549-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HeLa cells.

• 

  WB analysis of HepG2 using 20549-1-AP

  WB result of TOP2B antibody (20549-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells.

• 

  WB analysis of HepG2 using 20549-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20549-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20549-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20549-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 20549-1-AP

  IP Result of anti-TOP2B (IP:20549-1-AP, 4ug; Detection:20549-1-AP 1:300) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human cervical cancer using 20549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 20549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 20549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 20549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 20549-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (20549-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 20549-1-AP

  Immunofluorescent analysis of HepG2 cells, using TOP2B antibody 20549-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

20549-1-AP cible TOP2B dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: topoisomerase (DNA) II beta 180kDa
• Masse moléculaire calculée: 183 kDa
• Poids moléculaire observé: 183 kDa
• Numéro d’acquisition GenBank: NM_001068
• Symbole du gène: TOP2B
• Identification du gène (NCBI): 7155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B.

Publications

Species	Application	Title
mouse	IF	Science; Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.; Authors - Anand Minajigi
human	WB,IP	Mol Cell; MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".; Authors - Subhendu K Das
human	WB	J Med Chem; Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.; Authors - Zongyuan Zhou
human	WB	RNA; DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells; Authors - Maya David
mouse	WB	Sci Rep; Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy.; Authors - Fei Xu ; KD Validated
human		Sci Rep; Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4'-demethylepipodophyllotoxin.; Authors - Lin-Yang Sun