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Anticorps Polyclonal de lapin anti-TPH1

TPH1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 20879-1-AP

Synonymes

TPH, TPH1, TPRH, TRPH, Tryptophan 5 hydroxylase 1, Tryptophan 5 monooxygenase 1, tryptophan hydroxylase 1

Galerie de données de validation

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  • Western Blot (WB) analysis of SH-SY5Y cells using TPH1 Polyclonal antibody (20879-1-AP)

    WB analysis of SH-SY5Y using 20879-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20879-1-AP (TPH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules SH-SY5Y

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

20879-1-AP cible TPH1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène TPH1 Protéine recombinante Ag14978
Nom complet tryptophan hydroxylase 1
Masse moléculaire calculée 466 aa, 53 kDa
Poids moléculaire observé 51 kDa
Numéro d’acquisition GenBankBC106739
Symbole du gène TPH1
Identification du gène (NCBI) 7166
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tryptophan hydroxylase 1(TPH1) catalyzes the rate-limiting step in the synthesis of serotonin in the periphery. Recently, it has been shown that expression of the tryptophan hydroxylase 1 gene is increased in lungs and pulmonary endothelial cells from patients with idiopathic pulmonary arterial hypertension. It catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. The protein has low expression.

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