Anticorps Polyclonal de lapin anti-TPT1

• Phare
• Validé par KD/KO

TPT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10824-1-AP

Synonymes

Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1

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Galerie de données de validation

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  WB analysis of MCF-7 using 10824-1-AP

  WB result of TPT1 antibody (10824-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells.

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  WB analysis of mouse placenta using 10824-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10824-1-AP (TPT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10824-1-AP (TPT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 10824-1-AP

  IP Result of anti-TPT1 (IP:10824-1-AP, 4ug; Detection:10824-1-AP 1:500) with human placenta tissue lysate 1520ug.

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  IHC staining of human colon cancer using 10824-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10824-1-AP (TPT1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10824-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10824-1-AP (TPT1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10824-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TPT1 antibody (10824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules MCF-7, tissu placentaire de souris
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10824-1-AP cible TPT1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPT1 Protéine recombinante Ag1268
• Nom complet: tumor protein, translationally-controlled 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC003352
• Symbole du gène: TPT1
• Identification du gène (NCBI): 7178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22-25 kDa TPT1 in lysates. An additional band around 50 kDa can also be observed occasionally, possibly representing the dimer form of TPT1.

Publications

Species	Application	Title
human	WB	Pharmacol Res; Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect.; Authors - Dongrong Zhu
human,mouse	WB,IHC,IF	Int J Biol Sci; Ailanthus Altissima-derived Ailanthone enhances Gastric Cancer Cell Apoptosis by Inducing the Repression of Base Excision Repair by Downregulating p23 Expression.; Authors - Chun-Ming Wang
human	WB	Chem Biol Interact; AMG900 as novel inhibitor of the translationally controlled tumor protein.; Authors - Nicolas Fischer
human	IP	Invest New Drugs; A novel ligand of the translationally controlled tumor protein (TCTP) identified by virtual drug screening for cancer differentiation therapy.; Authors - Nicolas Fischer
rat	WB,IHC	BMC Cancer; Identification of proteins involved in neural progenitor cell targeting of gliomas.; Authors - Staflin Karin K
human	WB	Cancer Sci; LncRNA TPT1-AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression.; Authors - Weimin Wu ; KD Validated