Anticorps Recombinant de lapin anti-TRAF2

TRAF2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241899A2

N° de cat : 84581-3-RR

Synonymes

241899A2, E3 ubiquitin-protein ligase TRAF2, EC:2.3.2.27, RING-type E3 ubiquitin transferase TRAF2, TNF receptor-associated factor 2

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Galerie de données de validation

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  WB analysis using 84581-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84581-3-RR (TRAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84581-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84581-3-RR (TRAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84581-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84581-3-RR (TRAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 84581-3-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84581-3-RR (TRAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 84581-3-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84581-3-RR (TRAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84581-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (84581-3-RR, Clone: 241899A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 84581-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRAF2 Recombinant antibody (84581-3-RR, Clone:241899A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84581-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84581-3-RR against Human TRAF2 were performed. The affinity constant is 0.544 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C2C12, cellules HEK-293T, cellules HepG2
• Résultats positifs en IHC: human ovary cancer tissue, human rectal cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84581-3-RR cible TRAF2 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRAF2 Protéine recombinante Ag25387
• Nom complet: TNF receptor-associated factor 2
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC032410
• Symbole du gène: TRAF2
• Identification du gène (NCBI): 7186
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumor necrosis factor (TNF) receptor-associated factor-2 (TRAF2) is one of the members of the TRAF superfamily protein, which is an intracellular junction protein with E3 ligase activity. TRAF2 mediates and regulates the activation of nuclear factor kappa B (NF-κB) and microtubule-associated protein kinase (MAPK) signaling pathways by binding to TNFR family proteins. Recent studies have demonstrated that TRAF2 regulates tumor progression by through multiple pathways.

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