Anticorps Recombinant de lapin anti-TRAF6

TRAF6 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230430H3

N° de cat : 83535-2-RR

Synonymes

230430H3, E3 ubiquitin-protein ligase TRAF6, EC:2.3.2.27, Interleukin-1 signal transducer, RING-type E3 ubiquitin transferase TRAF6

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Galerie de données de validation

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  WB analysis using 83535-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83535-2-RR (TRAF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 83535-2-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83535-2-RR (TRAF6 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 83535-2-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83535-2-RR (TRAF6 antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83535-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83535-2-RR (TRAF6 antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83535-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83535-2-RR (TRAF6 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 83535-2-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug TRAF6 Recombinant antibody (83535-2-RR, Clone:230430H3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293T, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83535-2-RR cible TRAF6 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRAF6 Protéine recombinante Ag14858
• Nom complet: TNF receptor-associated factor 6
• Masse moléculaire calculée: 522 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC031052
• Symbole du gène: TRAF6
• Identification du gène (NCBI): 7189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

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